Calprotectin, Human, mAb 27E10 Antibody
Mouse monoclonal antibody to Calprotectin
Product Specifications
Product Name Alternative
S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein
Reactivity
Human
Cross Reactivity
Mouse - No, Rhesus monkey - Yes
Immunogen
Human blood monocytes
Clonality
Monoclonal
Conjugation
Unconjugated
Dilution
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: non-reduced; ~22 kDa; doesn€t recognize the single proteins S100A8 and S100A9 HPLC: reduced (~17 kDa) and non-reduced (various sizes due to association with other elements (Ref1) F: acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon) (Ref1) FC: Extracelullar expression on monocytes, as negative control HL-60, platelets, lymphocytes can be used.
Tested Applications
FC, IA, IF, IHC-Fr, IP, WB
Preservative
Quantities >500 μg will be sent without preservatives and are carrier-free.
Isotype
Mouse IgG1
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