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Calprotectin, Human, mAb 27E10 Antibody

Mouse monoclonal antibody to Calprotectin

Product Specifications

Product Name Alternative

S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein

Reactivity

Human

Cross Reactivity

Mouse - No, Rhesus monkey - Yes

Immunogen

Human blood monocytes

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

W: non-reduced; ~22 kDa; doesn€t recognize the single proteins S100A8 and S100A9 HPLC: reduced (~17 kDa) and non-reduced (various sizes due to association with other elements (Ref1) F: acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon) (Ref1) FC: Extracelullar expression on monocytes, as negative control HL-60, platelets, lymphocytes can be used.

Tested Applications

FC, IA, IF, IHC-Fr, IP, WB

Preservative

Quantities >500 μg will be sent without preservatives and are carrier-free.

Isotype

Mouse IgG1

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