OxiSelect™ Nε- (carboxyethyl) lysine (CEL) Competitive ELISA Kit

First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation, the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the predetermined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in the OxiSelect™ CEL ELISA Kit will not cross react with CML protein adducts.

Product Specifications

Overview

Provides rapid detection and quantitation of CEL protein adducts/Perform up to 5x96 assays, including standard curve and unknown protein samples/Will not cross react with CML protein adducts/

CAS Number

7732-18-5

UNSPSC

41116158

Hazard Statement

Excepted Quantities

Detection Method

Colorimetric

Features & Benefits

Provides rapid detection and quantitation of CEL protein adducts; Perform up to 5x96 assays, including standard curve and unknown protein samples; Will not cross react with CML protein adducts

Shipping Conditions

6 parts: 5 RT / 1 Blue Ice

Storage Conditions

Upon receipt, aliquot and store the CEL-BSA Standard, CEL Conjugate and 100X Conjugate Diluent at -20ºC to avoid multiple freeze/thaw cycles. Store all other kit components at 4ºC.

Classification

Oxidative Stress/ Damage->Protein Oxidation / Nitration->Advanced Glycation End Products->CEL (N-epsilon- (Carboxyethyl) Lysine) Assays and Reagents->CEL Competitive ELISA