VAP-1, Human, mAb 174-5 Antibody

For immunohistochemistry, and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

FC: Antibody 174-5 stains Ax cells stably transfected with VAP-1 cDNA. . As negative control mock transfected cells were used. (Ref.1) IHC-F:- Tissue sections were fixed in acetonebefore staining with 174-5. As positive control anti-VAP-1 mAb 2D10 was used and as negative control an isotype matched irrelevant mAb. (Ref.1) . FS: Antibody 174-5 inhibits lymphocyte infiltration in liver allograft rejection. . The antibody was administered intravenously at a concentration of 2 mg/kg. A irrelevant isotype-matched antibody served as a negative control. (Ref.1) .