CD73, Human, mAb 4G4 Antibody

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

1 ml (100 μg/ml) 0.2 um filtered protein G purified antibody solution in PBS containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.

F: Tissue sections were fixed in acetone.- As positive control anti-CD31 was used and as negative control an isotype antibody (Ref.5) . FC: Antibody 4G4 stains the extracellular domain of CD73. As positive control anti-CD3- was used and as negative control an irrelevant antibody (Ref.1) . FS: Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer (Ref.1) . Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes (Ref.5) and reduces enzyme activity (Ref.2) . IF: HUVEC cells were seeded on gelatin-coated coverslips and stained with antibody (Ref.5) .