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Beta2-adrenoceptor, Human, mAb 6H8 Antibody

Mouse monoclonal antibody to Beta2-adrenoceptor

Product Specifications

Reactivity

Human

Cross Reactivity

Guinea pig - Yes, Rat - Yes

Immunogen

Free peptide Beta2-H19C

Clonality

Monoclonal

Conjugation

Unconjugated

Dilution

For- flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

FC: A431 human epidermoid cells in suspension for 1 h at 4°C in PBS containing monoclonal Antibody 6H8 (260 nM) . After 1 h of incubation, the cells were fixed with 2% Formaldehyde (Ref.1) FS: Antibody 6H8 functions as an agonist in neonatal rat cardiomyocytes (Ref 1) . Furthermore, Fab fragments of agonist-like antibody 6H8 behave as antagonist. (Ref 2) . IA: The antibody against beta2-adrenoceptor is useful as detector in an ELISA setting (Ref1)

Tested Applications

FC, Functional Studies, IA

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin

Isotype

Mouse IgG1

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