TRACP5a, Human, mAb 220 Antibody
Mouse monoclonal antibody to TRACP5a
Product Specifications
Product Name Alternative
TRAP5a
Reactivity
Human
Immunogen
Recombinant TRACP
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
For immunohistochemistry, immunoprecipitation, immuno assays and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: A non-reduced and reduced sample treatment and SDS-Page was used. The band size is 35 kDa (Ref.1) . IHC-P: Tissue sections were pretreated with prior heat-induced antigen retrieval by incubation overnight at 72 °C in a commercial solution of EDTA at pH 8.0. (Ref.1) .
Tested Applications
IA, IP, PS, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG1
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