SIRL-1, Human, mAb 1A5 Antibody
Mouse monoclonal antibody to SIRL-1
Product Specifications
Product Name Alternative
V-set and transmembrane domain-containing protein 1, Signal inhibitory receptor on leukocytes-1
UniProt
Q6UX27
Reactivity
Human
Immunogen
Purified SIRL-1-hIg fusion protein in PBS
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immuno precipitation, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: A non-reduced sample treatment and SDS-Page was used. The band sizes are 45 kDa and fainter band of 37 kDa in transfected cells and 37 kDa in granulocytes (Ref.1) . FS: Antibody 1A5 showed inhibition of FcÎμRI-mediated degranulation by RBL-2H3 cells transfected with SIRL-1. The percentage of inhibition of degranulation by SIRL-1 was calculated by (OD405 TNP stimulation €" OD405 TNP with SIRL-1 crosslinking) / (OD405 TNP stimulation €" OD405 spontaneous release) x 100.
Tested Applications
FC, Functional Studies, IP, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
Isotype
Mouse IgG1
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