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SIRL-1, Human, mAb 1A5 Antibody

Mouse monoclonal antibody to SIRL-1

Product Specifications

Product Name Alternative

V-set and transmembrane domain-containing protein 1, Signal inhibitory receptor on leukocytes-1

UniProt

Q6UX27

Reactivity

Human

Immunogen

Purified SIRL-1-hIg fusion protein in PBS

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

For immuno precipitation, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

W: A non-reduced sample treatment and SDS-Page was used. The band sizes are 45 kDa and fainter band of 37 kDa in transfected cells and 37 kDa in granulocytes (Ref.1) . FS: Antibody 1A5 showed inhibition of FcÎμRI-mediated degranulation by RBL-2H3 cells transfected with SIRL-1. The percentage of inhibition of degranulation by SIRL-1 was calculated by (OD405 TNP stimulation €" OD405 TNP with SIRL-1 crosslinking) / (OD405 TNP stimulation €" OD405 spontaneous release) x 100.

Tested Applications

FC, Functional Studies, IP, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Isotype

Mouse IgG1

Frequently Asked Questions

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