Prion PrPsc, mAb 1.5D7 Antibody
Mouse monoclonal antibody to Prion PrPSC
Product Specifications
Product Name Alternative
Spongiform encephalopathies
Cross Reactivity
Bovine - Yes, Human - Yes, Mouse - Yes
Immunogen
BoPrP153-165 (GSDYEDRYYRENM)
Clonality
Monoclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
W: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1) . IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1) .
Tested Applications
IA, IHC-Fr, PS, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Isotype
Mouse IgG2b
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