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Prion PrPsc, mAb 1.5D7 Antibody

Mouse monoclonal antibody to Prion PrPSC

Product Specifications

Product Name Alternative

Spongiform encephalopathies

Cross Reactivity

Bovine - Yes, Human - Yes, Mouse - Yes

Immunogen

BoPrP153-165 (GSDYEDRYYRENM)

Clonality

Monoclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

For immunohistochemistry, and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

W: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1) . IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1) .

Tested Applications

IA, IHC-Fr, PS, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.

Isotype

Mouse IgG2b

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