ICAM-1, Human, pAb Antibody
Rabbit polyclonal antibody to ICAM-1
Product Specifications
Reactivity
Human
Cross Reactivity
Baboon - Yes, Chimpanzee - Yes, Cynomolgus monkey - Yes, Rhesus monkey - Yes
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 μg/ml) in tissue culture medium at 37 °C.
Form
1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
IA, IHC-Fr, IP, WB
Preservative
0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Isotype
Rabbit Ig
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