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ICAM-1, Human, pAb Antibody

Rabbit polyclonal antibody to ICAM-1

Product Specifications

Reactivity

Human

Cross Reactivity

Baboon - Yes, Chimpanzee - Yes, Cynomolgus monkey - Yes, Rhesus monkey - Yes

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 μg/ml) in tissue culture medium at 37 °C.

Form

1 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

IA, IHC-Fr, IP, WB

Preservative

0.2 ml (100 μg/ml) 0.2 μm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Isotype

Rabbit Ig

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