Anti-Fibrillarin (Mouse Monoclonal)
Mouse monoclonal antibody to Fibrillarin
Product Specifications
Reactivity
Other
Immunogen
Raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes.
Clonality
Monoclonal
Clone
B50H5
Conjugation
Unconjugated
Concentration
100ug/100ul
Dilution
For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL) . For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL) . For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluorescence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
Tested Applications
ICC, IF, WB
Host or Source
Mouse
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
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