Anti-GFP Tag (Goat Polyclonal)
Goat polyclonal antibody to GFP Tag
Product Specifications
Reactivity
Other
Immunogen
The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) . No reaction was observed against Human, Mouse or Rat serum proteins.
Concentration
100ug/100ul
Dilution
ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) . No reaction was observed against Human, Mouse or Rat serum proteins.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
Tested Applications
ELISA, ICC, IF, IHC, IP, WB
Host or Source
Goat
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
Isotype
IgG
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