Anti-NF-kB p65 (Phospho-Thr254)
Rabbit polyclonal antibody to NF-kB p65 (Phospho-Thr254)
Product Specifications
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P) .
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Concentration
1ug/ul
Dilution
WB: 1:500~1:1000; IHC: 1:50~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: 1:500~1:1000; IHC: 1:50~1:100
Tested Applications
IHC, WB
Host or Source
Rabbit
Preservative
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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