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Rat (Wistar) Serum

Product Specifications

Background

As a qualitative serum with an optimized protein content to study the normal protein composition of rat serum. To identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference serum in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference serum does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western immunoblotting and electroimmunodiffusion (Laurell), expressing the results obtained with the serum sample under investigation as a percentage of the protein concentration in the normal reference serum. For a standard rat serum with assigned values for IgA, IgM and the subclasses of IgG see NOR-03.

Species Reactivity

Rat

Type

Serum

Field of Research

Veterinary

Assay Principle

Normal serum protein reference sera are shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be stored frozen.

Form

Delipidated, lyophilized, stable reference serum; No preservative added

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

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