Ethyl methanesulfonate

Ethyl methanesulfonate is an orally active biochemical agent. Ethyl methanesulfonate induces Apoptosis. Ethyl methanesulfonate acts on DNA, alkylating it and causing changes in DNA structure, which in turn triggers a series of biological effects such as mutation and cell death. Ethyl methanesulfonate induces kidney and nervous system tumors. Ethyl methanesulfonate is widely used in the field of genetic toxicology research and is often used to induce gene mutations in organisms to study gene function, the mechanism of genetic diseases, and the effects of environmental mutagenic factors, etc[1][2][3][4][5][6][7][8][9][10].

Product Specifications

CAS Number

[62-50-0]

UNSPSC

12352200

Hazard Statement

H302, H315, H317, H319, H340, H350, H361

Target

Apoptosis; Biochemical Assay Reagents; DNA Alkylator/Crosslinker

Type

Biochemical Assay Reagents

Related Pathways

Apoptosis; Cell Cycle/DNA Damage; Others

Field of Research

Cancer; Neurological Disease

Assay Protocol

https://www.medchemexpress.com/ethyl-methanesulfonate.html

Concentration

10mM

Purity

99.99

Solubility

DMSO : ≥ 100 mg/mL

Smiles

CS(=O)(OCC)=O

Molecular Formula

C3H8O3S

Molecular Weight

124.15

Precautions

H302, H315, H317, H319, H340, H350, H361

References & Citations

[1]Stopper H, et al. Induction of micronuclei in human cell lines and primary cells by combination treatment with gamma-radiation and ethyl methanesulfonate. Mutagenesis. 2002 Mar;17 (2) :177-81.|[2]Morris SM, et al. The role of programmed cell death in the toxicity of the mutagens, ethyl methanesulfonate and N-ethyl-N'-nitrosourea, in AHH-1 human lymphoblastoid cells. Mutat Res. 1994 Apr 1;306 (1) :19-34.|[3]Kojima H, et al. Combined mutagenicity of methyl methanesulfonate and ethyl methanesulfonate in Chinese hamster V79 cells. Mutat Res. 1992 Apr;266 (2) :171-80.|[4]Ray SD, et al. Role of cellular energy status in tocopheryl hemisuccinate cytoprotection against ethyl methanesulfonate-induced toxicity. Arch Biochem Biophys. 1994 May 15;311 (1) :180-90.|[5]Stavreva DA, et al. Single cell gel electrophoresis analysis of genomic damage induced by ethyl methanesulfonate in cultured tobacco cells. Mutat Res. 1998 Dec 3;422 (2) :323-30.|[6]Lavé T, et al. In vivo and in vitro characterization of ethyl methanesulfonate pharmacokinetics in animals and in human. Toxicol Lett. 2009 Nov 12;190 (3) :303-9.|[7]Kondo K, et al. Micronucleus test with ethyl methanesulfonate administered by intraperitoneal injection and oral gavage. Mutat Res. 1989 Aug;223 (4) :373-5.|[8]Itoh S, et al. PIGRET assay can detect mutagenicity of ethyl methanesulfonate much earlier than RBC Pig-a assay. Mutat Res Genet Toxicol Environ Mutagen. 2016 Nov 15;811:102-105.|[9]Swann PF, et al. Induction of rat kidney tumours by ethyl methanesulphonate and nervous tissue tumours by methyl methanesulphonate and ethyl methanesulphonate. Nature. 1969 Aug 30;223 (5209) :947-9.|[10]Sega GA. Unscheduled DNA synthesis in the germ cells of male mice exposed in vivo to the chemical mutagen ethyl methanesulfonate. Proc Natl Acad Sci U S A. 1974 Dec;71 (12) :4955-9.