Anti-PhosphoTyrosine (pY)
Rabbit polyclonal antibody to PhosphoTyrosine (pY)
Product Specifications
Reactivity
Human
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphotyrosine conjugated KLH.
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Affinity Purified
Concentration
1ug/ul
Dilution
This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Purity
Affinity Purified
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This affinity-purified antibody is suitable for use in ELISA, western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphothreonine or phosphoserine residues. The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Tested Applications
ELISA, IHC, IP, WB
Host or Source
Rabbit
Preservative
PBS, pH 7.4 with 0.05% sodium azide.
Isotype
IgG
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