Chikungunya virus E protein ectodomain (CHIKV-E)

Product Specifications

Background

Chikungunya virus (CHIKV) belongs to the Semliki Forest antigenic group of the genus Alphaviridae, which includes other arthritogenic alphaviruses, such as o’nyong-nyong, Ross River, Barmah Forest, and Mayaro viruses.Its genome is closely related to that of o’nyong-nyong virus, and consists of a single 11.8-kbp strand of positive sense RNA, which encodes a 2472 amino acid nonstructural and a 1244 amino acid structural polyprotein.3 The polyproteins give rise to the four nonstructural proteins (nsP1-4) that make up the viral replication machine, and five structural proteins. Each spherical viral particle is approximately 70 nm in diameter and is comprised of a strand of genomic RNA, encapsidated by capsid (C) proteins, surrounded by a host cell–derived lipid bilayer spiked with heterodimers of envelope proteins E1 and E2.4 The other two structural proteins, 6K and E3, are leader peptides for E1 and E2, respectively, and are not observed in abundance in the mature virion.

Structure Composition

Inclusion body;0.5mol Urea, PH=8

Swiss Prot

ABN04192.1

Reactivity

E. coli Expression

Applications

Reseach

Dilution

Reseach

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Molecular Weight

1095bp;40kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Carrier:pET30a-CHIKV-E-His (C-term) Virus:structural polyprotein [Chikungunya virus]

Host or Source

His-tag

Control Type

Chikungunya virus E protein ectodomain, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.