CD300A Recombinant Protein

Product Specifications

Background

CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail. Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granulocytes, mast cells, and subsets of B cells. Human, but not mouse, CD300a is also expressed by unstimulated NK cells and subsets of T cells. Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation.

Swiss Prot

Q9UGN4

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~23kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

CAS Number

9000-83-3

AA Sequence

LSKCRTVAGPVGGSLSVQCPYEKEHRTLNKYWCRPPQIFLCDKIVETKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDFHDPVVEVEVSVFPASTSMTPASITAAKTSTITTAFPPVSSTTLFAVGATHSASIQEETEEVVNSQLP