CD158d Recombinant Protein

Product Specifications

Background

NKAT (NK-associated transcripts) gene products, known as killer immunoglobulin-like receptors or KIRs, downregulate the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. This family of receptors is characterized by an extracellular region with two to three immunoglobulin-superfamily domains and a cytoplasmic domain with an antigen receptor activation motif (ARAM) . KIRs and other inhibitory receptors also possess a common cytoplasmic sequence (I/VxYxxL/V) known as an ITIM (immunoreceptor tyrosine-based inhibitory motif) . The human inhibitory human killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4), also referred to as 2DL4 or CD158d, triggers potent IFN-γ responses but weak cytotoxicity in resting NK cells because of the low stoichiometric association with γ

CAS Number

9000-83-3

Swiss Prot

Q99706

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~28kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

TGGGCACATGTTGGTGGTCAGGATAAACCGTTCTGCAGCGCATGGCCGAGCGCCGTTGTTCCGCAGGGTGGTCATGTGACCCTGCGTTGTCATTATCGTCGTGGCTTCAATATCTTCACCCTGTATAAAAAAGACGGCGTTCCGGTTCCGGAACTGTATAATCGTATCTTCTGGAATAGCTTCCTGATTAGCCCGGTGACCCCGGCCCATGCAGGCACATATCGTTGCCGCGGCTTCCATCCGCATAGCCCGACCGAATGGAGCGCACCGAGCAATCCGCTGGTGATTATGGTTACCGGCCTGTATGAAAAACCGAGTCTGACCGCCCGTCCGGGTCCGACAGTGCGTGCAGGTGAAAATGTGACCCTGAGTTGTAGCAGCCAGAGTAGCTTCGATATCTATCATCTGAGTCGTGAAGGTGAAGCCCATGAACTGCGTCTGCCGGCAGTTCCGAGCATTAATGGCACCTTCCAGGCAGACTTCCCGCTGGGTCCGGCAACCCATGGTGAAACCTATCGCTGCTTCGGTAGCTTCCATGGTAGCCCGTATGAATGGAGCGATCCGAGCGATCCGCTGCCGGTGAGCGTGACCGGTAATCCGAGTAGCAGTTGGCCGAGCCCGACCGAGCCGAGCTTCAAAACCGGCATTGCCCGCCATCTGCAT