CD133 Recombinant Protein

Product Specifications

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

CAS Number

9000-83-3

Swiss Prot

O43490

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~31kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

GGTGCAAATGTGGAAAAACTGATCTGTGAACCGTATACCAGTAAAGAACTGTTCCGCGTTCTGGATACCCCGTATCTGCTGAATGAAGATTGGGAATATTATCTGAGTGGTAAACTGTTCAATAAGAGTAAAATGAAGCTGACCTTCGAACAGGTGTATAGCGATTGTAAAAAAAATCGCGGTACCTATGGCACCCTGCATCTGCAGAATAGCTTCAATATTAGTGAACATCTGAACATTAACGAGCATACCGGCAGTATTAGCAGCGAACTGGAAAGTCTGAAAGTGAATCTGAATATCTTCCTGCTGGGCGCAGCCGGTCGTAAAAATCTGCAGGACTTCGCCGCCTGCGGCATTGATCGTATGAATTATGATAGTTATCTGGCCCAGACCGGCAAAAGTCCGGCCGGCGTTAATCTGCTGAGCTTCGCATACGATCTGGAAGCAAAAGCCAATAGCCTGCCGCCGGGCAATCTGCGCAATAGCCTGAAACGCGATGCCCAGACCATTAAAACCATTCATCAGCAGCGCGTGCTGCCGATTGAACAGAGCCTGAGCACCCTGTATCAGAGCGTTAAAATTCTGCAGCGTACCGGCAATGGCCTGCTGGAACGCGTTACCCGTATTCTGGCCAGCCTGGACTTCGCCCAGAACTTCATTACCAATAATACCAGTAGTGTTATCATCGAAGAAACCAAAAAATACGGCCGTACCATTATTGGCTACTTCGAACATTATCTGCAGTGGATTGAATTCAGTATTAGTGAAAAAGTGGCAAGCTGCAAACCGGTGGCCACCGCCCTGGATACCGCAGTGGATGTGTTCCTGTGTAGCTATATTATTGATCCGCTGAAT