CD226/DNAM-1 Recombinant Protein

Product Specifications

Background

DNAM-1/CD226 is a member of the immunoglobulin-like superfamily. It is expressed on T cells, natural killer (NK) cells, monocytes, and macrophages. It is a major activating receptor on NK cells. Engagement with its ligands Nectin-2 (CD112) and PVR (poliovirus receptor, also known as CD155) on the target cells leads to downstream activation of ERK, AKT, and PLCg2 signaling pathways that are crucial for NK activation and NK-mediated killing. LFA-1 physically associates with DNAM-1/CD226 and contributes to its signaling. Activating signal of DNAM-1/CD226 is counterbalanced by inhibitory receptors TIGIT and CD96, competing for the common ligands CD122 and PVR.

CAS Number

9000-83-3

Swiss Prot

Q15762

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~26kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

GAAGAAGTTCTGTGGCATACCAGTGTTCCGTTCGCAGAAAATATGAGTCTGGAATGCGTGTATCCGAGTATGGGTATTCTGACCCAGGTGGAATGGTTCAAAATTGGTACCCAGCAGGATAGCATTGCAATCTTCAGTCCGACCCATGGTATGGTGATTCGCAAACCGTATGCAGAACGTGTGTACTTCCTGAATAGCACCATGGCCAGCAATAATATGACCCTGTTCTTCCGCAATGCCAGTGAAGATGATGTTGGCTATTATAGCTGTAGCCTGTATACCTATCCGCAGGGCACCTGGCAGAAAGTGATTCAGGTGGTTCAGAGTGATAGCTTCGAAGCAGCAGTTCCGAGCAATAGCCATATTGTGAGCGAACCGGGTAAAAATGTGACCCTGACCTGTCAGCCGCAGATGACCTGGCCGGTGCAGGCAGTTCGCTGGGAAAAAATTCAGCCGCGCCAGATTGATCTGCTGACCTATTGCAATCTGGTGCATGGCCGTAACTTCACCAGTAAATTCCCGCGTCAGATTGTGAGTAATTGTAGTCATGGCCGTTGGAGCGTTATTGTGATTCCGGATGTGACCGTTAGCGATAGTGGCCTGTATCGCTGTTATCTGCAGGCAAGCGCAGGCGAAAATGAAACCTTCGTGATGCGCCTGACCGTTGCAGAAGGTAAAACCGATAATCAGTATACCCTGTTCGTGGCA