CD22 Recombinant Protein

Product Specifications

Background

CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction) . CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR) . After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellular carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation. CD22 has been actively pursued as a therapeutic target for autoimmune diseases. Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for multiple B cell malignancies.

CAS Number

9000-83-3

Swiss Prot

P20273

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~74kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

GATAGTAGCAAATGGGTGTTCGAACATCCGGAAACCTTATATGCCTGGGAAGGTGCATGCGTGTGGATTCCGTGTACCTATCGCGCACTGGATGGCGATCTGGAAAGCTTCATTCTGTTCCATAATCCGGAATATAATAAGAATACCAGCAAATTCGACGGCACCCGCCTGTATGAAAGCACCAAAGATGGTAAAGTTCCGAGTGAACAGAAACGTGTTCAGTTCCTGGGCGATAAAAATAAAAATTGCACCCTGAGCATTCATCCGGTGCATCTGAATGATAGCGGTCAGCTGGGCCTGCGCATGGAAAGTAAAACCGAAAAATGGATGGAACGTATTCATCTGAATGTTAGCGAACGTCCGTTCCCGCCGCATATTCAGCTGCCGCCGGAAATTCAGGAAAGTCAGGAAGTTACCCTGACCTGTCTGCTGAACTTCAGTTGCTATGGCTATCCGATTCAGCTGCAGTGGCTGCTGGAAGGCGTGCCGATGCGCCAGGCCGCTGTGACAAGCACCAGCCTGACCATTAAAAGTGTGTTCACCCGTAGTGAACTGAAATTCAGTCCGCAGTGGAGCCATCATGGTAAAATTGTGACCTGCCAGCTGCAGGATGCCGATGGCAAATTCCTGAGCAATGATACCGTTCAGCTGAATGTGAAACATACCCCGAAACTGGAAATTAAAGTGACCCCGAGTGATGCAATTGTTCGCGAAGGTGATAGTGTGACCATGACCTGTGAAGTTAGCAGCAGTAATCCGGAATACACCACCGTTAGCTGGCTGAAAGATGGCACCAGTCTGAAAAAACAGAATACCTTCACCCTGAATCTGCGCGAAGTGACCAAAGATCAGAGCGGTAAATATTGCTGTCAGGTTAGCAATGATGTGGGCCCGGGCCGTAGCGAAGAAGTGTTCCTGCAGGTGCAGTATGCACCGGAACCGAGTACCGTGCAGATTCTGCATAGCCCGGCCGTGGAAGGTAGCCAGGTTGAATTCCTGTGCATGAGCCTGGCAAATCCGCTGCCGACCAATTATACCTGGTATCATAATGGCAAAGAAATGCAGGGTCGCACCGAAGAAAAAGTGCATATTCCGAAAATTCTGCCGTGGCATGCAGGTACCTATAGTTGTGTGGCCGAAAATATTCTGGGTACCGGTCAGCGCGGTCCGGGTGCTGAACTGGATGTGCAGTATCCGCCGAAAAAAGTGACCACCGTTATTCAGAATCCGATGCCGATTCGTGAAGGCGATACCGTGACCCTGAGCTGTAATTATAATAGCAGCAATCCGAGCGTGACCCGCTATGAATGGAAACCGCATGGCGCCTGGGAAGAACCGAGTCTGGGTGTGCTGAAAATTCAGAATGTGGGCTGGGATAATACCACCATTGCATGTGCCGCATGCAATAGCTGGTGTAGCTGGGCCAGCCCGGTTGCACTGAATGTTCAGTATGCACCTCGTGATGTGCGTGTGCGTAAAATTAAACCGCTGAGCGAAATTCATAGTGGTAATAGTGTTAGTCTGCAGTGTGACTTCAGCAGTAGCCATCCGAAAGAAGTGCAGTTCTTCTGGGAAAAAAATGGTCGCCTGCTGGGCAAAGAAAGTCAGCTGAACTTCGATAGTATTAGCCCGGAAGATGCCGGTAGCTATAGTTGTTGGGTTAATAATAGCATTGGTCAGACCGCCAGTAAAGCATGGACCCTGGAAGTGCTGTATGCACCGCGTCGTCTGCGTGTGAGTATGAGTCCGGGTGATCAGGTTATGGAAGGTAAAAGTGCAACCCTGACCTGCGAAAGTGATGCAAATCCGCCGGTTAGTCATTATACCTGGTTCGATTGGAATAATCAGAGTCTGCCGTATCATAGTCAGAAACTGCGCCTGGAACCGGTTAAAGTGCAGCATAGCGGTGCATATTGGTGTCAGGGCACCAATAGTGTTGGTAAAGGCCGTAGCCCGCTGAGTACCCTGACCGTGTATTATAGCCCGGAAACCATTGGCCGTCGC