CD14 Protein

Product Specifications

Background

CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage.

Swiss Prot

P08571

Modification Site

NdeI-XhoI

Expression System

Pet-22b (+)

Tag

His-tag

Purity

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE) .

Solubility

PBS, 4M Urea, PH7.4

Molecular Weight

~40kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Host or Source

E.coli

AA Sequence

ACCACCCCTGAACCGTGCGAACTGGATGATGAAGACTTCCGCTGCGTGTGTAACTTCAGTGAACCGCAGCCGGATTGGAGCGAAGCCTTCCAGTGCGTTAGTGCAGTTGAAGTGGAAATTCATGCAGGCGGCCTGAATCTGGAACCGTTCCTGAAACGCGTTGATGCAGATGCAGATCCGCGCCAGTATGCCGATACCGTTAAAGCCCTGCGTGTGCGTCGTCTGACCGTGGGTGCAGCACAGGTGCCGGCACAGCTGCTGGTTGGTGCACTGCGTGTTCTGGCCTATAGTCGCCTGAAAGAACTGACCCTGGAAGATCTGAAAATTACCGGTACCATGCCGCCGCTGCCGCTGGAAGCCACCGGTCTTGCACTGAGCAGCCTGCGTCTGCGTAATGTGAGCTGGGCCACCGGTCGCAGCTGGCTGGCAGAACTGCAGCAGTGGCTGAAACCGGGTCTGAAAGTTCTGAGCATTGCCCAGGCACATAGCCCGGCATTCAGTTGCGAACAGGTTCGCGCCTTCCCGGCACTGACCAGTCTGGATCTGAGTGATAATCCGGGTCTGGGCGAACGCGGTCTGATGGCCGCACTGTGTCCGCATAAATTCCCGGCAATTCAGAATCTGGCACTGCGTAATACCGGTATGGAAACACCTACCGGTGTGTGTGCAGCCCTGGCCGCCGCTGGTGTGCAGCCTCATAGCCTGGATCTGTCACATAATAGCCTGCGCGCCACCGTTAATCCGAGCGCCCCGCGTTGCATGTGGAGCAGCGCACTGAATAGCCTGAATCTGAGCTTCGCAGGTCTGGAACAGGTTCCGAAAGGCCTGCCGGCAAAACTGCGTGTTCTTGATCTGAGCTGTAATCGCCTGAATCGCGCACCGCAGCCGGACGAACTGCCGGAAGTGGATAATCTGACCCTGGATGGTAATCCGTTCCTGGTGCCGGGCACCGCACTGCCTCATGAAGGCAGCATGAAT