Tau-441 (2N4R) Wild-Type Pre-formed Fibrils

Human Recombinant Tau-441 (2N4R) Wild-Type PFFs (Baculovirus/Sf9)

Product Specifications

Background

Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in adult brain yet is absent from the fetal brain (1) . Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (2) . Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies (3) . Sf9/Baculovirus-expressed Tau 2N4R Pre-Formed Fibrils are generated without heparin.

Product Name Alternative

MAPT, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, 2N4R

UNSPSC

12352202

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Swiss Prot

P10636-8

Expression System

Baculovirus/Sf9

Host

Baculovirus (Sf9)

Origin Species

Human

Target

Tau-441 (2N4R) Wild-Type

Conjugation

No Tag

Nature

Recombinant

Sequence

MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Applications

WB | SDS PAGE | In vitro Assay

Field of Research

Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau

Purification Method

Ion-exchange Purified

Purification

Ion-exchange Purified

Limit Of Detection

Protein certified >95% pure on SDS-PAGE & Nanodrop analysis. Made with low endotoxin monomer.

Concentration

2 mg/ml

Purity

>95%

Weight

0.02

Length

Full Length (1-441 aa)

Buffer

1X PB pH7.4

Molecular Weight

45.84 kDa

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

Additionnal Information

For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or the product datasheet for further information. Monomer source is catalog# SPR-496.

References & Citations

1. Goedert et al. 1989. Multiple Isoforms of Human Microtubule-associated Protein Tau: Sequences and Localization in Neurofibrilary Tangles of Alzheimer’s Disease. Neuron. doi: 10.1016/0896-6273(89)90210-9. 2. Iqbal K., Liu F., and Gong C.X. 2016. Tau and neurodegenerative disease: The story so far. Nat. Rev. Neurol. DOI: 10.1038/nrneurol.2015.225 3. Tepper et al. 2014. Oligomer Formation of Tau Protein Hyperphophorylated in Cells. The Journal of Biological Chemistry, DOI 10.1074/jbc.M114.611368

Shipping Conditions

Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.

Storage Conditions

-80ºC

Notes

Protein Length

Full Length (1-441 aa)

Background Reference 01

1. Goedert et al. 1989. Multiple Isoforms of Human Microtubule-associated Protein Tau: Sequences and Localization in Neurofibrilary Tangles of Alzheimer’s Disease. Neuron. doi: 10.1016/0896-6273 (89) 90210-9. 2. Iqbal K., Liu F., and Gong C.X. 2016. Tau and neurodegenerative disease: The story so far. Nat. Rev. Neurol. DOI: 10.1038/nrneurol.2015.225 3. Tepper et al. 2014. Oligomer Formation of Tau Protein Hyperphophorylated in Cells. The Journal of Biological Chemistry, DOI 10.1074/jbc.M114.611368