RecombinantTNF-α, Human (P.pastoris-expressed)

Tumor Necrosis Factor-Alpha (TNF-alpha) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) produced in Pichia pastoris is a glycosylated polypeptide chain of 157 amino acids. A fully biologically active molecule, rhTNF-alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

<1.0 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE analysis.

Bioactivity

ED50<0.08ng/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >1.25 x 10ˆ7units/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

17.4kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Tumor Necrosis Factor-Alpha (rhTNF-alpha) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Host or Source

P. pastoris

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

VRSSSR TPSDKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA L