RecombinantEnterokinase, Porcine
Enterokinase (EK) is a specific protease that cleaves a protein after lysine residue at its recognition site: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by proline. If a fusion tag is placed at the N-terminus with an enterokinase site, enterokinase will be able to remove the fusion tag and to generate the protein exactly as you need without adding any unwanted residues. GenScript's Porcine Enterokinase is a highly purified recombinant porcine enterokinase (Light Chain) expressed from P. Pastoris GS115 and is supplied in two forms for customers: a. Liquid in 50% glycerol; b. Lyophilized (glycerol free) .
Product Specifications
CAS Number
9000-83-3
Bioactivity
Reconstitution
Reconstituted in ddH2O or PBS at 100 μg/ml.
Molecular Weight
Theoretical MW: 21,880 Da; The apparent MW on SDS-PAGE: about 40,000 Da.
Storage Conditions
Store at -20°C after receiving. Reconstituted EK is stable for 24 hours at 2°C - 8°C. For long term storage, store it at -20°C.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Host or Source
P. pastoris
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
0
Frequently Asked Questions
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