RecombinantEnterokinase, LightChain, Porcine

GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.

Product Specifications

CAS Number

9000-83-3

Bioactivity

One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Reconstitution

Reconstituted in ddH2O or PBS at 100 μg/ml.

Molecular Weight

Theoretical MW: 21,880 DaThe apparent MW on SDS-PAGE: about 40,000 Da

Storage Conditions

Store at -20°C after delivery.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.