RecombinantShh, Mouse

Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are threerelatedHh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) . They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical) . Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant mouseSonic Hedgehog (rmShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 176 amino acids. A fully biologically active molecule, rmShh, is obtained by proprietary chromatographic techniques at GenScript with a molecular mass of 19.8kDa analyzed by reducing SDS-PAGE.

Product Specifications

CAS Number

9000-83-3

Endotoxin

<0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Bioactivity

ED50<1.0μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of>1.0x 10ˆ3units/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

19.8kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant mouseSonic Hedgehog (rmShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmShhshould be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

IVI GPGRGFGKRR HPKKLTPLAY KQFIPNVAEK TLGASGRYEG KITRNSERFK ELTPNYNPDI IFKDEENTGA DRLMTQRCKD KLNALAISVM NQWPGVKLRV TEGWDEDGHH SEESLHYEGR AVDITTSDRD RSKYGMLARL AVEAGFDWVY YESKAHIHCS VKAENSVAAK SGG