RecombinantPDGF-AA, Mouse

Platelet-Derived Growth Factor-AA (PDGF-AA) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In chemical terms, platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB) . The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types, and their effects are mediated through two distinct receptors termed α and β. Differences exist in isoform binding to each receptor. Ingeneral, PDGF isoforms are potent mitogens for connective tissue cells including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial andendothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. PDGF-AA plays a significant role in blood vessel formation (angiogenesis) .Recombinant Mouse Platelet-Derived Growth Factor-AA (PDGF-AA) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 125 amino acids each. A fully biologically active molecule, rmPDGF-AA has a molecular mass of 28.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% as analyzed by SDS-PAGE.

Bioactivity

ED50 <50 ng/, measured in a cell proliferation assay using 3T3 cells.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

28.7 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant Mouse Platelet-Derived Growth Factor-AA (PDGF-AA) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, recombinant Mouse PDGF-AA should be stable up to 1 week at 4°C or up to 2 months at -20°C.