RecombinantNT-4, Human
Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Product Specifications
CAS Number
9000-83-3
Endotoxin
< 0.3 EU/μg, determined by LAL method.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Bioactivity
ED50 < 5.0 μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0x 10ˆ2 units/mg.
Reconstitution
Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml.
Molecular Weight
28.1 kDa, a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers.
Storage Conditions
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against 50mM acetic acid.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA
Available Sizes
Frequently Asked Questions
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