RecombinantNANOG-TAT, Human

NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.Recombinant human NANOG-TAT (rhNANOG-TAT) produced in E.coli is a single chain, 318 amino acids non-glycosylated polypeptide. A fully biologically active molecule, rhNANOG-TAT has a molecular mass of 36.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Reconstitution

Reconstituted in ddH2O or PBS at 100 μg/ml.

Molecular Weight

36.2 kDa, analyzed by reducing SDS-PAGE.

Storage Conditions

Recombinant human NANOG-TAT (rhNANOG-TAT) remains stable up to 1-2 weeks at 4°C from date of receipt. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freezing and thawing cycles.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Sterile Filtered solution contains 10mM PB, 300mM NaCl, pH7.4.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

MSVDPACPQ SLPCFEASDC KESSPMPVIC GPEENYPSLQ MSSAEMPHTE TVSPLPSSMD LLIQDSPDSS TSPKGKQPTS AENSVAKKED KVPVKKQKTR TVFSSTQLCV LNDRFQRQKY LSLQQMQELS NILNLSYKQV KTWFQNQRMK SKRWQKNNWP KNSNGVTQKA SAPTYPSLYS SYHQGCLVNP TGNLPMWSNQ TWNNSTWSNQ TQNIQSWSNH SWNTQTWCTQ SWNNQAWNSP FYNCGEESLQ SCMQFQPNSP ASDLEAALEA AGEGLNVIQQ TTRYFSTPQT MDLFLNYSMN MQPEDVGGYG RKKRRQRRR