RecombinantM-CSF, Human
Macrophage Colony-Stimulating Factor 1 (M-CSF), involved especially in monocytopoiesis, [1]is a hematopoietic growth factor. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail[2]. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain[3], and it is only active in the disulfide-linked dimeric form[4], which is bonded at Cys63.Recombinant human Macrophage Colony-Stimulating Factor 1 (rhM-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 159 amino acids each. A fully biologically active molecule, rhM-CSF has a molecular mass of 28 kDaanalyzed by non-reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Product Specifications
CAS Number
9000-83-3
Endotoxin
<1 EU/μg, determined by LAL method.
Purity
> 95% as analyzed by non-reducing SDS-PAGE.
Bioactivity
ED50 of 1 - 3 ng/ml, measured by cell proliferation assay of M-NFS-60, corresponding to a specific activity of 3.3 x 10ˆ5-1 x 10ˆ6 units/mg.
Reconstitution
Reconstituted in ddH2O or PBS or Tris-HCl, pH 8.0 at 100 μg/ml.
Molecular Weight
28 kDa, observed by non-reducing SDS-PAGE.
Storage Conditions
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.
AA Sequence
Available Sizes
Frequently Asked Questions
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