RecombinantIL-6, Human

Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.Recombinant human Interleukin-6 (rhIL-6) produced in E.coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE analysis.

Bioactivity

ED50 <0.1ng/ml, measured by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells, corresponding to a specific activity of > 1 x 10ˆ7 units/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

20.9 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized after extensive dialysis against 1xPBS.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

MPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM