RecombinantIGF-I, Human

Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which is differentially expressed by various tissues. Mature human IGF-I respectively shares 94% and 96% aa sequence identity with mouse and rat IGF-I. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but IGF-II can alone bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor) . IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults. Recombinant human Insulin-like growth factor I (rhIGF-I) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhIGF-I has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Bioactivity

ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0x 10ˆ5 units/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

7.7 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Insulin-like growth factor I (rhIGF-I) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIGF-I should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.