RecombinantGM-CSF, Human

Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities [4].Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells [5].Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E.coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

<1 EU/μg, determined by LAL method.

Purity

>95 % as analyzed by SDS-PAGE.

Bioactivity

ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of >2.0 x 10ˆ6units/mg

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

14 kDa, observed by SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.