RecombinantG-CSF, Human

Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF) . In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.

Product Specifications

CAS Number

9000-83-3

Endotoxin

<0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Bioactivity

ED50 <0.1ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of >1.0x 10ˆ7 IU/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

18.8kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized after extensive dialysis against 25mM Tris, pH8.0.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

MTPLGPASS LPQSFLLKCL EQVRKIQGDG AALQEKLCAT YKLCHPEELV LLGHSLGIPW APLSSCPSQA LQLAGCLSQL HSGLFLYQGL LQALEGISPE LGPTLDTLQL DVADFATTIW QQMEELGMAP ALQPTQGAMP AFASAFQRRA GGVLVASHLQ SFLEVSYRVL RHLAQP