RecombinantFGF-4, Human

Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E.coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.

Product Specifications

CAS Number

9000-83-3

Endotoxin

<0.2 EU/μg, determined by LAL method.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Bioactivity

ED50<0.5ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of >2.0x 10ˆ6 units/mg.

Reconstitution

Reconstituted in ddH2O at 100 μg/ml.

Molecular Weight

19.4kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH7.5.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. For research use only.

AA Sequence

MAPTAPNGT LEAELERRWE SLVALSLARL PVAAQPKEAA VQSGAGDYLL GIKRLRRLYC NVGIGFHLQA LPDGRIGGAH ADTRDSLLEL SPVERGVVSI FGVASRFFVA MSSKGKLYGS PFFTDECTFK EILLPNNYNA YESYKYPGMF IALSKNGKTK KGNRVSPTMK VTHFLPRL