RecombinantENA78/CXCL5 (9-78a.a.), Human

Epithelial cellderived neutrophilactivating peptide (ENA78) also known as C-X-C motif chemokine 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties Full length CXCL5 (78 a.a.) is cleaved at the Nterminal end by cathepsin G and chymotrypsin to ENA-74 (74 a.a.) and ENA-70 (70a.a.), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor.Recombinant human ENA-78/CXCL5 (9-78a.a.) produced in E.coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhENA-78/CXCL5 (9-78a.a.) has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Product Specifications

CAS Number

9000-83-3

Endotoxin

< 0.2 EU/μg, determined by LAL method.

Purity

> 95% as analyzed by SDS-PAGE.

Bioactivity

The EC50 value of human ENA78/CXCL5 (9-78 a.a.) on Caˆ2+ mobilization assay in CHO-K1/G15/hCXCR2 cells (human G15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 50 ng/ml.

Reconstitution

Reconstituted in ddH2O or PBS at 100 μg/ml.

Molecular Weight

7.7 kDa, observed by reducing SDS-PAGE.

Storage Conditions

Lyophilized recombinant human ENA78/CXCL5 (9-78a.a.) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human ENA78/CXCL5 (9-78) should be stable up to 1 week at 4°C or up to 2 months at -20°C.