Recombinant Murine Macrophage Inflammatory Protein-3 beta/CCL19 (rMuMIP-3b/CCL19)

Macrophage Inflammatory Protein-3 beta also called CCL19, ELC (EBI1 Ligand Chemokine), Exodus-3 is a reported β chemokine that binds specifically to the chemokine receptor CCR7 / EBI1 / BLR2. It is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells. MIP-3 beta is a chemoattractant for T and B lymphocytes and myeloid progenitor cells.

Product Specifications

CAS Number

9000-83-3

Endotoxin

Less than 1EU/μg of rMuMIP-3b/CCL19 as determined by LAL method.

Purity

>95% by SDS-PAGE and HPLC analyses.

Bioactivity

Measured by its ability to chemoattract human CCR7 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 3-15 ng/mL.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.

Molecular Weight

Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles.