Recombinant Human Melanoma Inhibitor Activity Protein 2 (rHuMIA-2)

MIA2 is a secreted cytokine and a member of the MIA/OTOR family. Members of this family which also includes MIA, OTOR, and TANGO share a Src homology-3 (SH3) -like domain. MIA2 is predominantly expressed in hepatocytes. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity.

Product Specifications

CAS Number

9000-83-3

Endotoxin

Less than 1EU/µg of rHuMIA-2 as determined by LAL method.

Purity

>97% by SDS-PAGE and HPLC analyses.

Bioactivity

Data Not Available.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Molecular Weight

11.5 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.