Recombinant Human Eotaxin-3/CCL26 (rHuEotaxin-3/CCL26)
Eotaxin3, also named CCL26 or SCYA26, is a novel human CC chemokine coded by CXCL26 gene at chromosome 7 in human. Recombinant Eotaxin3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. Recombinant Eotaxin3/CCL26 is chemotactic for eosinophils and PHAactivated T cells. Eotaxin3/CCL26 induces calcium flux in eosinophils as well as in CCR3 transfected cells. Eotaxin3/CCL26 has also been shown to crossdesensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3 transfected cell line.
Product Specifications
CAS Number
9000-83-3
Endotoxin
Less than 1EU/μg of rHuEotaxin-3/CCL26 as determined by LAL method.
Purity
>97% by SDS-PAGE and HPLC analyses.
Bioactivity
Measured by its ability to chemoattract mouse CCR3 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.3-0.5μg/mL.
Reconstitution
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 71 amino acid residues.
Storage Conditions
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Host or Source
Escherichia coli.
Recommended Usage
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
AA Sequence
TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L
Available Sizes
Frequently Asked Questions
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