What is the catalog number for Recombinant Human MCP-2 (rHu MCP-2/CCL8)?

The catalog number for Recombinant Human MCP-2 (rHu MCP-2/CCL8) is PR1087.

What sizes are available for Recombinant Human MCP-2 (rHu MCP-2/CCL8)?

Recombinant Human MCP-2 (rHu MCP-2/CCL8) is available in the following size(s): 1 mg, 2 µg, 10 µg.

How many pack sizes does Recombinant Human MCP-2 (rHu MCP-2/CCL8) come in?

Recombinant Human MCP-2 (rHu MCP-2/CCL8) is available in 3 different pack sizes.

Is Recombinant Human MCP-2 (rHu MCP-2/CCL8) in stock?

Recombinant Human MCP-2 (rHu MCP-2/CCL8) is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Human MCP-2 (rHu MCP-2/CCL8)

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the CC family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1.MCP-3 also shares 58% amino acid identity with MCP-2. Similarly to other CC chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Product Specifications

CAS Number

9000-83-3

Endotoxin

Less than 1EU/mg of rHuMCP-2/CCL8 as determined by LAL method.

Purity

>96% by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED50 for this effect is typically 0.03-0.1μg/mL, corresponding to a Specific Activity of > 1 x 104 IU/mg.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Molecular Weight

8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.