What is the catalog number for Recombinant Human Interleukin-33 (rHuIL-33)?

The catalog number for Recombinant Human Interleukin-33 (rHuIL-33) is PR1071.

What sizes are available for Recombinant Human Interleukin-33 (rHuIL-33)?

Recombinant Human Interleukin-33 (rHuIL-33) is available in the following size(s): 1 mg, 2 µg, 10 µg.

How many pack sizes does Recombinant Human Interleukin-33 (rHuIL-33) come in?

Recombinant Human Interleukin-33 (rHuIL-33) is available in 3 different pack sizes.

Is Recombinant Human Interleukin-33 (rHuIL-33) in stock?

Recombinant Human Interleukin-33 (rHuIL-33) is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Human Interleukin-33 (rHuIL-33)

IL33, also known as NFHEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription. IL33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL1α or IL1β stimulation. IL-33 shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.

Product Specifications

CAS Number

9000-83-3

Endotoxin

Less than 1EU/mg of rHuIL-33 as determined by LAL method.

Purity

>97% by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤0.05ng/ml, corresponding to a specific activity of ≥2x107units/mg.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Molecular Weight

Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.

Storage Conditions

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, 150mM NaCl, 1mM EDTA, 2mM β-Mercaptoethanol, pH7.4.

Host or Source

Escherichia coli.

Recommended Usage

This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China

AA Sequence

MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVLLSYYESQHPS NE SGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEKPLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCTENILFKLSET