Rabbit Anti-Sheep IgG (H+L) Rhodamine (TRITC)
Fluorophore: Tetramethyl rhodamine 5 (and6) -isothiocyanate (TRITC) Amax=550nm; Emax=570nm Fluorophore/ProteinAbsorbanceRatio: A550/A280=0.5
Product Specifications
Background
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG.It also reacts with the light chains of other sheeep immunoglobuins. No antibody was detected against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human, and rabbit serum proteins. The antibody may cross-react with immunoglobulins from other species.
CAS Number
9007-83-4
Immunogen
Sheep IgG (H+L)
Conjugation
Rhodamine (TRITC)
Applications
IF FCM
Concentration
1 mg/ml
Dilution
1:50 -1:200 for most applications
Purity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, containing 1% BSA,50% glycerol
Storage Conditions
Store at 4°C. Avoid freeze-thaw cycles. Prepare working dileuion fresh each day.
Shelf Life
Six months from date of reconstitution.
Host or Source
Rabbit
Preservative
0.05% Sodium Azide
Classification
Secondary Antibodies
Available Sizes
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