Rabbit Anti-Goat IgG (H+L) HRP
Peroxidase-conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)
Product Specifications
Background
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This product exhibits minimal inherent cross-reactivity to mouse serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse, human, and rabbit serum proteins, but the antibody may cross-react with immunoglobulins from other species.
CAS Number
9007-83-4
Immunogen
Goat IgG (H+L)
Conjugation
Peroxidase (HRP)
Applications
WB ELISA IHC ICC
Concentration
1 mg/ml
Dilution
1:500 -1:5000 for immunohisto/cytochemistry 1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000-1:200,000 for Western blotting with ECL substrates
Purity
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, containing 1% BSA,50% glycerol
Storage Conditions
Store at 4°C. Avoid freeze-thaw cycles. Prepare working dileuion fresh each day.
Shelf Life
One year from date of reconstitution.
Host or Source
Rabbit
Preservative
0.05% Sodium Azide
Classification
Secondary Antibodies
Available Sizes
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