RAG2 Recombinant Rabbit mAb
Product Specifications
Background
Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V (D) J recombination. V (D) J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V (D) J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps.
CAS Number
9000-83-3
Structure Composition
Store at -20°C. Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Product Name Alternative
Rag-2
Swiss Prot
P21784
Reactivity
Mouse
Immunogen
A synthetic peptide of mouse RAG2
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:1000-1:5000
Purity
Affinity Purification
Modification
Unmodification
Molecular Weight
59 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Host or Source
Rabbit
Available Sizes
Curated Selection
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