RNA polymerase II CTD repeat YSPTSPS Recombinant Rabbit mAb

Product Specifications

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

CAS Number

9000-83-3

Structure Composition

Store at -20°C. Supplied in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Product Name Alternative

RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Swiss Prot

P24928

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human RNA polymerase II CTD repeat YSPTSPS

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, FC, IP

Purity

Affinity Purification

Modification

Unmodification

Molecular Weight

250 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IgG

Applications Notes

Western blot detection of RNA polymerase II CTD repeat YSPTSPS in Hela, A549, MCF-7, U251, HL-60 cell lysates using RNA polymerase II CTD repeat YSPTSPS antibody (1:1000 diluted) .