Phospho-c-Jun-S243 polyclonal antibody

Product Specifications

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

AP-1; AP1; c-Jun; JUN; c

Swiss Prot

P05412

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S243 of human Jun (NP_002219.1) .

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

Refer to figures

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phosphorylated Antibodies

Applications Notes

Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-c-Jun-S243 Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.