RAB5B polyclonal antibody
Product Specifications
Background
Protein transport. Probably involved in vesicular traffic.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
RAB5B
Swiss Prot
P61020
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human RAB5B (NP_002859.1) .
Conjugation
Unconjugated
Applications
WB, IF/ICC
Dilution
WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
24kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Polyclonal Antibodies
Applications Notes
Western blot analysis of extracts of various cell lines, using RAB5B antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Host or Source
Rabbit
Available Sizes
Curated Selection
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