RAB5B polyclonal antibody

Product Specifications

Background

Protein transport. Probably involved in vesicular traffic.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RAB5B

Swiss Prot

P61020

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human RAB5B (NP_002859.1) .

Conjugation

Unconjugated

Applications

WB, IF/ICC

Dilution

WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

24kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Polyclonal Antibodies

Applications Notes

Western blot analysis of extracts of various cell lines, using RAB5B antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.